pegfpc1 fusion mammalian protein expression vector Search Results


vector pegfp c1  (Addgene inc)
95
Addgene inc vector pegfp c1
Vector Pegfp C1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfpc1+fusion+mammalian+protein+expression+vector/pEGFP(C1)-RepoMan+(Plasmid+%2344212)/pmc11849630-199-17-21
Average 95 stars, based on 1 article reviews
vector pegfp c1 - by Bioz Stars, 2026-09
95/100 stars
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pegfp c1 nphp5  (Addgene inc)
93
Addgene inc pegfp c1 nphp5
Pegfp C1 Nphp5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfpc1+fusion+mammalian+protein+expression+vector/pEGFP(C1)-NIPP1+(Plasmid+%2344221)/pmc11104825-120-11-18
Average 93 stars, based on 1 article reviews
pegfp c1 nphp5 - by Bioz Stars, 2026-09
93/100 stars
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the mammalian expression vector pegfp-c1  (Promega)
90
Promega the mammalian expression vector pegfp-c1
The Mammalian Expression Vector Pegfp C1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfpc1+fusion+mammalian+protein+expression+vector/gfp+expressing+plasmid+pegfp+c1/pm19054385-113-37-41
Average 90 stars, based on 1 article reviews
the mammalian expression vector pegfp-c1 - by Bioz Stars, 2026-09
90/100 stars
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pegfp-c1  (Becton Dickinson)
90
Becton Dickinson pegfp-c1
Pegfp C1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfpc1+fusion+mammalian+protein+expression+vector/pegfp+c1/pmc03162436-242-28-29
Average 90 stars, based on 1 article reviews
pegfp-c1 - by Bioz Stars, 2026-09
90/100 stars
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gfp  (Addgene inc)
93
Addgene inc gfp
(A) Immunoblots for ER, PR, and HER2 in different human breast cancer cell lines. ER + (MCF-7 and T-47D) and ER - (MDA-MB-231) breast cancer cell lines were prepared for western blotting. The expression pattern of different hormone receptors was determined. Protein expression levels were compared to expression levels of α-tubulin; each value under the blots indicates relative protein expression levels determined by densitometric analysis, unless specifically mentioned (mean (SD); n = 4). Dots denote ER + (MCF-7 and T-47D) or ER - (MDA-MB-231) breast cancer cells treated with different concentrations of (B) TAM and (C) β-estradiol (E 2 ). Bars denote (D) ER + MCF-7, (E) ER + T-47D, and (F) ER - MDA-MB-231 breast cancer cells treated with TAM after silencing with NC or CNSK1G2 siRNA. (G) ER - MDA-MB-231 also transiently transfected <t>with</t> <t>GFP-C1</t> or GFP-ERα plasmid DNA as well as with NC or CSNK1G1 siRNA. Twenty-four hours after transfection, cells were treated with vehicle or 1 μM TAM for 24 h. Cellular toxicity was then measured using the MTT assay (mean (SD); n = 4; * P < 0.05, ** P < 0.01, # P < 0.001 vs . each represented counterpart).
Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfpc1+fusion+mammalian+protein+expression+vector/pEGFP-C1-ER+alpha+(Plasmid+%2328230)/pmc08051802-83-9-13
Average 93 stars, based on 1 article reviews
gfp - by Bioz Stars, 2026-09
93/100 stars
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pmcherry c1  (Addgene inc)
94
Addgene inc pmcherry c1
(A) Immunoblots for ER, PR, and HER2 in different human breast cancer cell lines. ER + (MCF-7 and T-47D) and ER - (MDA-MB-231) breast cancer cell lines were prepared for western blotting. The expression pattern of different hormone receptors was determined. Protein expression levels were compared to expression levels of α-tubulin; each value under the blots indicates relative protein expression levels determined by densitometric analysis, unless specifically mentioned (mean (SD); n = 4). Dots denote ER + (MCF-7 and T-47D) or ER - (MDA-MB-231) breast cancer cells treated with different concentrations of (B) TAM and (C) β-estradiol (E 2 ). Bars denote (D) ER + MCF-7, (E) ER + T-47D, and (F) ER - MDA-MB-231 breast cancer cells treated with TAM after silencing with NC or CNSK1G2 siRNA. (G) ER - MDA-MB-231 also transiently transfected <t>with</t> <t>GFP-C1</t> or GFP-ERα plasmid DNA as well as with NC or CSNK1G1 siRNA. Twenty-four hours after transfection, cells were treated with vehicle or 1 μM TAM for 24 h. Cellular toxicity was then measured using the MTT assay (mean (SD); n = 4; * P < 0.05, ** P < 0.01, # P < 0.001 vs . each represented counterpart).
Pmcherry C1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfpc1+fusion+mammalian+protein+expression+vector/pmCherry-C1+mCherry-NLS+(Plasmid+%2358476)/pmc11849630-199-20-21
Average 94 stars, based on 1 article reviews
pmcherry c1 - by Bioz Stars, 2026-09
94/100 stars
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pegfpc1  (ATeam Scientific)
90
ATeam Scientific pegfpc1
(A) Immunoblots for ER, PR, and HER2 in different human breast cancer cell lines. ER + (MCF-7 and T-47D) and ER - (MDA-MB-231) breast cancer cell lines were prepared for western blotting. The expression pattern of different hormone receptors was determined. Protein expression levels were compared to expression levels of α-tubulin; each value under the blots indicates relative protein expression levels determined by densitometric analysis, unless specifically mentioned (mean (SD); n = 4). Dots denote ER + (MCF-7 and T-47D) or ER - (MDA-MB-231) breast cancer cells treated with different concentrations of (B) TAM and (C) β-estradiol (E 2 ). Bars denote (D) ER + MCF-7, (E) ER + T-47D, and (F) ER - MDA-MB-231 breast cancer cells treated with TAM after silencing with NC or CNSK1G2 siRNA. (G) ER - MDA-MB-231 also transiently transfected <t>with</t> <t>GFP-C1</t> or GFP-ERα plasmid DNA as well as with NC or CSNK1G1 siRNA. Twenty-four hours after transfection, cells were treated with vehicle or 1 μM TAM for 24 h. Cellular toxicity was then measured using the MTT assay (mean (SD); n = 4; * P < 0.05, ** P < 0.01, # P < 0.001 vs . each represented counterpart).
Pegfpc1, supplied by ATeam Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfpc1+fusion+mammalian+protein+expression+vector/pegfpc1/pmc05486644-24-38-42
Average 90 stars, based on 1 article reviews
pegfpc1 - by Bioz Stars, 2026-09
90/100 stars
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pgex 4t 2  (Cytiva Europe)
95
Cytiva Europe pgex 4t 2
(A) Immunoblots for ER, PR, and HER2 in different human breast cancer cell lines. ER + (MCF-7 and T-47D) and ER - (MDA-MB-231) breast cancer cell lines were prepared for western blotting. The expression pattern of different hormone receptors was determined. Protein expression levels were compared to expression levels of α-tubulin; each value under the blots indicates relative protein expression levels determined by densitometric analysis, unless specifically mentioned (mean (SD); n = 4). Dots denote ER + (MCF-7 and T-47D) or ER - (MDA-MB-231) breast cancer cells treated with different concentrations of (B) TAM and (C) β-estradiol (E 2 ). Bars denote (D) ER + MCF-7, (E) ER + T-47D, and (F) ER - MDA-MB-231 breast cancer cells treated with TAM after silencing with NC or CNSK1G2 siRNA. (G) ER - MDA-MB-231 also transiently transfected <t>with</t> <t>GFP-C1</t> or GFP-ERα plasmid DNA as well as with NC or CSNK1G1 siRNA. Twenty-four hours after transfection, cells were treated with vehicle or 1 μM TAM for 24 h. Cellular toxicity was then measured using the MTT assay (mean (SD); n = 4; * P < 0.05, ** P < 0.01, # P < 0.001 vs . each represented counterpart).
Pgex 4t 2, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfpc1+fusion+mammalian+protein+expression+vector/PGEX-4T-2/pmc05473537-40-8-9
Average 95 stars, based on 1 article reviews
pgex 4t 2 - by Bioz Stars, 2026-09
95/100 stars
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plasmid pfn21ahyg-egfp  (Promega)
90
Promega plasmid pfn21ahyg-egfp
(A) Immunoblots for ER, PR, and HER2 in different human breast cancer cell lines. ER + (MCF-7 and T-47D) and ER - (MDA-MB-231) breast cancer cell lines were prepared for western blotting. The expression pattern of different hormone receptors was determined. Protein expression levels were compared to expression levels of α-tubulin; each value under the blots indicates relative protein expression levels determined by densitometric analysis, unless specifically mentioned (mean (SD); n = 4). Dots denote ER + (MCF-7 and T-47D) or ER - (MDA-MB-231) breast cancer cells treated with different concentrations of (B) TAM and (C) β-estradiol (E 2 ). Bars denote (D) ER + MCF-7, (E) ER + T-47D, and (F) ER - MDA-MB-231 breast cancer cells treated with TAM after silencing with NC or CNSK1G2 siRNA. (G) ER - MDA-MB-231 also transiently transfected <t>with</t> <t>GFP-C1</t> or GFP-ERα plasmid DNA as well as with NC or CSNK1G1 siRNA. Twenty-four hours after transfection, cells were treated with vehicle or 1 μM TAM for 24 h. Cellular toxicity was then measured using the MTT assay (mean (SD); n = 4; * P < 0.05, ** P < 0.01, # P < 0.001 vs . each represented counterpart).
Plasmid Pfn21ahyg Egfp, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfpc1+fusion+mammalian+protein+expression+vector/plasmid+pfn21ahyg+egfp/10__1074_slash_jbc__m111__291625-51-0-28
Average 90 stars, based on 1 article reviews
plasmid pfn21ahyg-egfp - by Bioz Stars, 2026-09
90/100 stars
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bst  (OriGene)
96
OriGene bst
(A) Immunoblots for ER, PR, and HER2 in different human breast cancer cell lines. ER + (MCF-7 and T-47D) and ER - (MDA-MB-231) breast cancer cell lines were prepared for western blotting. The expression pattern of different hormone receptors was determined. Protein expression levels were compared to expression levels of α-tubulin; each value under the blots indicates relative protein expression levels determined by densitometric analysis, unless specifically mentioned (mean (SD); n = 4). Dots denote ER + (MCF-7 and T-47D) or ER - (MDA-MB-231) breast cancer cells treated with different concentrations of (B) TAM and (C) β-estradiol (E 2 ). Bars denote (D) ER + MCF-7, (E) ER + T-47D, and (F) ER - MDA-MB-231 breast cancer cells treated with TAM after silencing with NC or CNSK1G2 siRNA. (G) ER - MDA-MB-231 also transiently transfected <t>with</t> <t>GFP-C1</t> or GFP-ERα plasmid DNA as well as with NC or CSNK1G1 siRNA. Twenty-four hours after transfection, cells were treated with vehicle or 1 μM TAM for 24 h. Cellular toxicity was then measured using the MTT assay (mean (SD); n = 4; * P < 0.05, ** P < 0.01, # P < 0.001 vs . each represented counterpart).
Bst, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfpc1+fusion+mammalian+protein+expression+vector/pCMV6-XL5+Mammalian+Expression+Vector/pm20529266-216-1-8
Average 96 stars, based on 1 article reviews
bst - by Bioz Stars, 2026-09
96/100 stars
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Image Search Results


(A) Immunoblots for ER, PR, and HER2 in different human breast cancer cell lines. ER + (MCF-7 and T-47D) and ER - (MDA-MB-231) breast cancer cell lines were prepared for western blotting. The expression pattern of different hormone receptors was determined. Protein expression levels were compared to expression levels of α-tubulin; each value under the blots indicates relative protein expression levels determined by densitometric analysis, unless specifically mentioned (mean (SD); n = 4). Dots denote ER + (MCF-7 and T-47D) or ER - (MDA-MB-231) breast cancer cells treated with different concentrations of (B) TAM and (C) β-estradiol (E 2 ). Bars denote (D) ER + MCF-7, (E) ER + T-47D, and (F) ER - MDA-MB-231 breast cancer cells treated with TAM after silencing with NC or CNSK1G2 siRNA. (G) ER - MDA-MB-231 also transiently transfected with GFP-C1 or GFP-ERα plasmid DNA as well as with NC or CSNK1G1 siRNA. Twenty-four hours after transfection, cells were treated with vehicle or 1 μM TAM for 24 h. Cellular toxicity was then measured using the MTT assay (mean (SD); n = 4; * P < 0.05, ** P < 0.01, # P < 0.001 vs . each represented counterpart).

Journal: PLoS ONE

Article Title: CSNK1G2 differently sensitizes tamoxifen-induced decrease in PI3K/AKT/mTOR/S6K and ERK signaling according to the estrogen receptor existence in breast cancer cells

doi: 10.1371/journal.pone.0246264

Figure Lengend Snippet: (A) Immunoblots for ER, PR, and HER2 in different human breast cancer cell lines. ER + (MCF-7 and T-47D) and ER - (MDA-MB-231) breast cancer cell lines were prepared for western blotting. The expression pattern of different hormone receptors was determined. Protein expression levels were compared to expression levels of α-tubulin; each value under the blots indicates relative protein expression levels determined by densitometric analysis, unless specifically mentioned (mean (SD); n = 4). Dots denote ER + (MCF-7 and T-47D) or ER - (MDA-MB-231) breast cancer cells treated with different concentrations of (B) TAM and (C) β-estradiol (E 2 ). Bars denote (D) ER + MCF-7, (E) ER + T-47D, and (F) ER - MDA-MB-231 breast cancer cells treated with TAM after silencing with NC or CNSK1G2 siRNA. (G) ER - MDA-MB-231 also transiently transfected with GFP-C1 or GFP-ERα plasmid DNA as well as with NC or CSNK1G1 siRNA. Twenty-four hours after transfection, cells were treated with vehicle or 1 μM TAM for 24 h. Cellular toxicity was then measured using the MTT assay (mean (SD); n = 4; * P < 0.05, ** P < 0.01, # P < 0.001 vs . each represented counterpart).

Article Snippet: pGFP-C1-ERα (GFP-ERα), a mammalian expression of ERα fused to GFP, was purchased from Addgene (#28230; Watertown, MA, USA).

Techniques: Western Blot, Expressing, Transfection, Plasmid Preparation, MTT Assay

Immunoblots for PI3K/AKT/mTOR/S6K and ERK signaling-associated proteins in (A-D) MCF-7, ( E-H) MDA-MB-231, and (I-K) GFP-ERα-overexpressed MDA-MB-231 cells. Western blotting analysis from the breast cells transfected with control siRNA ( NC ) or CSNK1G2 siRNA ( CSNK1G2 ) were performed after treatment with vehicle or 1 μM TAM for 24 h. Protein expression levels based on individual bands of phosphor were compared to expression levels of α-tubulin and/or pan (total) protein. Each value under the blots indicates relative protein expression levels determined by densitometric analysis. Data are presented as mean (SD); n = 4.

Journal: PLoS ONE

Article Title: CSNK1G2 differently sensitizes tamoxifen-induced decrease in PI3K/AKT/mTOR/S6K and ERK signaling according to the estrogen receptor existence in breast cancer cells

doi: 10.1371/journal.pone.0246264

Figure Lengend Snippet: Immunoblots for PI3K/AKT/mTOR/S6K and ERK signaling-associated proteins in (A-D) MCF-7, ( E-H) MDA-MB-231, and (I-K) GFP-ERα-overexpressed MDA-MB-231 cells. Western blotting analysis from the breast cells transfected with control siRNA ( NC ) or CSNK1G2 siRNA ( CSNK1G2 ) were performed after treatment with vehicle or 1 μM TAM for 24 h. Protein expression levels based on individual bands of phosphor were compared to expression levels of α-tubulin and/or pan (total) protein. Each value under the blots indicates relative protein expression levels determined by densitometric analysis. Data are presented as mean (SD); n = 4.

Article Snippet: pGFP-C1-ERα (GFP-ERα), a mammalian expression of ERα fused to GFP, was purchased from Addgene (#28230; Watertown, MA, USA).

Techniques: Western Blot, Transfection, Control, Expressing

(A) Cytotoxicity assay of either GFP-C1 or GFP-ERα-transfected ER - breast cancer cells. Bar denote relative percentage of % survival of transfected MDA-MB-231 cells after treatment with vehicle, 1 μM TAM, 2 μM D4476, and 1 μM TAM plus 2 μM D4476 for 24h. Cellular toxicity was measured using the MTT assay (mean (SD), n = 5; * P < 0.05, ** P < 0.01 vs . each represented counterpart). (B) Western blotting analysis from GFP-ERα-transfected ER - breast cells treated with vehicle or 2 μM D4476 together with 1 μM TAM for 24 h were performed. Immunoblots for PI3K/AKT/mTOR/S6K signaling-associated proteins in ER - MDA-MB-231 cells. Protein expression levels based on individual bands of phosphor were compared to expression levels of α-tubulin and/or pan (total) protein. Each value under the blots indicates relative protein expression levels determined by densitometric analysis (mean ± SEM, n = 4).

Journal: PLoS ONE

Article Title: CSNK1G2 differently sensitizes tamoxifen-induced decrease in PI3K/AKT/mTOR/S6K and ERK signaling according to the estrogen receptor existence in breast cancer cells

doi: 10.1371/journal.pone.0246264

Figure Lengend Snippet: (A) Cytotoxicity assay of either GFP-C1 or GFP-ERα-transfected ER - breast cancer cells. Bar denote relative percentage of % survival of transfected MDA-MB-231 cells after treatment with vehicle, 1 μM TAM, 2 μM D4476, and 1 μM TAM plus 2 μM D4476 for 24h. Cellular toxicity was measured using the MTT assay (mean (SD), n = 5; * P < 0.05, ** P < 0.01 vs . each represented counterpart). (B) Western blotting analysis from GFP-ERα-transfected ER - breast cells treated with vehicle or 2 μM D4476 together with 1 μM TAM for 24 h were performed. Immunoblots for PI3K/AKT/mTOR/S6K signaling-associated proteins in ER - MDA-MB-231 cells. Protein expression levels based on individual bands of phosphor were compared to expression levels of α-tubulin and/or pan (total) protein. Each value under the blots indicates relative protein expression levels determined by densitometric analysis (mean ± SEM, n = 4).

Article Snippet: pGFP-C1-ERα (GFP-ERα), a mammalian expression of ERα fused to GFP, was purchased from Addgene (#28230; Watertown, MA, USA).

Techniques: Cytotoxicity Assay, Transfection, MTT Assay, Western Blot, Expressing